divalent metal transporter 1  (Proteintech)

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Abnormal expression of antioxidant and iron metabolism-related proteins in liver samples of rats with chronic cholestasis. Control group was fed a chow diet, whereas the ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) Changes in ferroptosis antioxidant-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (B) Nrf2, (C) Keap1, (D) XCT, (E) HO-1 and (F) GPX4. (G) Changes in iron metabolism-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (H) TFR1, (I) FPN1, (J) DMT1, (K) Steap3 and (L) FTH1. Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001. ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine; Nrf2, nuclear factor erythroid-2-related factor 2; Keap1, Kelch-like ECH-associated protein 1, XCT, cystine/glutamate transporter; HO-1, heme oxygenase 1; GPX4, glutathione peroxidase 4; TFR1, transferrin receptor 1; FPN1, ferroportin 1; DMT1, divalent metal transporter 1; Steap3, six-transmembrane epithelial antigen of the prostate 3; FTH1, ferritin heavy chain 1.

Article Snippet: Ferroportin 1 (FPN1; cat. no. 26601-1-AP) and divalent metal transporter 1 (DMT1; cat. no. 20507-1-AP) antibodies were purchased from Proteintech Group, Inc. Ferritin heavy chain 1 (FTH1; cat. no. PA4412) and cystine/glutamate transporter (XCT; cat. no. T57046 ) antibodies were purchased from Abmart Pharmaceutical Technology Co., Ltd.

Techniques: Expressing, Control, Derivative Assay, Membrane

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Diagram of the mechanism of ferroptosis. ASCL4, acyl-CoA synthetase long-chain family member 4; COX2, cyclooxygenase 2; DMT1, divalent metal transporter 1; FPN, ferroportin; GSSG, oxidized glutathione; GPX4, glutathione peroxidase 4; GSH, glutathione; HO-1, heme oxygenase 1; Keap1, Kelch-like ECH-associated protein 1; LIP, labile iron pool; LPCAT3, lysophosphatidylcholine acyltransferase 3; NCOA4, nuclear receptor coactivator 4; NOX1, nicotinamide adenine dinucleotide phosphate oxidase 1; NQO1, NAD(P)H quinone dehydrogenase 1; Nrf2, nuclear factor erythroid-2-related factor 2; PL-PUFA-OOH, phospholipid-polyunsaturated fatty acid hydroperoxide; SLC7A11, solute carrier family 7 member 11; Steap3, six-transmembrane epithelial antigen of the prostate 3; TFR1, transferrin receptor 1.

Article Snippet: Ferroportin 1 (FPN1; cat. no. 26601-1-AP) and divalent metal transporter 1 (DMT1; cat. no. 20507-1-AP) antibodies were purchased from Proteintech Group, Inc. Ferritin heavy chain 1 (FTH1; cat. no. PA4412) and cystine/glutamate transporter (XCT; cat. no. T57046 ) antibodies were purchased from Abmart Pharmaceutical Technology Co., Ltd.

Techniques:

Journal: Journal of nanobiotechnology

Article Title: Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage.

doi: 10.1186/s12951-025-03149-9

Figure Lengend Snippet: Scheme 1 W-POM NCs for managing intracerebral haemorrhage. Schematic illustration of W-POM NCs inhibiting ferroptosis by modulating the S100A8/ A9-mediated iron metabolism pathway. The nanoclusters targeted oxidative stress and iron dysregulation to manage ICH, demonstrating their potential for neuroprotection and therapeutic efficacy. The figures were created using BioRender.com. ICH, intracerebral haemorrhage; W-POM NCs, tungsten- based polyoxometalate nanoclusters; TFR1, Transferrin Receptor 1; DMT1, Divalent Metal Transporter 1; FPN, Ferroportin; TLR4, Toll-like receptor 4; ROS, Reactive Oxygen Species

Article Snippet: The following primary antibodies were detected via WB: rabbit anti-transferrin receptor 1 (TFR1) polyclonal antibody (1:1000, AF5343, Affbiotech, China), rabbit anti-FPN polyclonal antibody (1:1000, NBP1-21502, Novus, US), rabbit anti-divalent metal transporter 1 (DMT1) polyclonal antibody (1:1000, 20507-1-AP, Proteintech, US), rabbit anti-S100A8/ A9 monoclonal antibody (1:1000, ab288715, Abcam, UK), rabbit anti-hepcidin monoclonal antibody (1:200, ab190775, Abcam, UK), anti-TLR4 polyclonal antibody (1:1000, AF7017, Affbiotech, China).

Techniques: Drug discovery

Journal: Journal of nanobiotechnology

Article Title: Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage.

doi: 10.1186/s12951-025-03149-9

Figure Lengend Snippet: Fig. 5 W-POM NCs inhibit ICH-induced neuroinflammation, oxidative stress and ferroptosis in ICH mice model. (a) Protein expression of iron transport- related proteins (TFR1, FPN, DMT1) assessed using western blot. (b-d) Statistical graph of grayscale values of TFR1, FPN, DMT1 protein expression. (e) Representative TEM images of mitochondrial morphology of the neurons in the perihaematomal area at 72 h after ICH. Red arrows indicate mitochondria. Representative images by Nissl staining (f) and FJB staining (g) in the perihaematomal tissue showing the effects of W-POM NCs on ICH-induced neuronal injury and neurodegeneration. Statistical analysis of Nissl staining (h) and FJB staining (i) results in the perihaematomal tissue. (j) Representative images of immunofluorescent staining for Iba-1 (red) and DAPI (blue) in the perihaematomal area at 72 h after ICH. (l) Quantitative analyses of Iba-1 positive cells in the perihaematomal area at 72 h after ICH. (k) Representative images of immunofluorescent staining for GFAP (red) and DAPI (blue) in the perihaemato mal area at 72 h after ICH. (m) Quantitative analyses of GFAP-positive cells in the perihaematomal area at 72 h after ICH. (*P < 0.05, **P < 0.01, ***P < 0.001, means ± SEM). ICH, intracerebral haemorrhage; TEM, transmission electron microscopy; W-POM NCs, tungsten-based polyoxometalate nanoclusters; TFR1, transferrin receptor 1; DMT1, divalent metal transporter 1; FPN, ferroportin

Article Snippet: The following primary antibodies were detected via WB: rabbit anti-transferrin receptor 1 (TFR1) polyclonal antibody (1:1000, AF5343, Affbiotech, China), rabbit anti-FPN polyclonal antibody (1:1000, NBP1-21502, Novus, US), rabbit anti-divalent metal transporter 1 (DMT1) polyclonal antibody (1:1000, 20507-1-AP, Proteintech, US), rabbit anti-S100A8/ A9 monoclonal antibody (1:1000, ab288715, Abcam, UK), rabbit anti-hepcidin monoclonal antibody (1:200, ab190775, Abcam, UK), anti-TLR4 polyclonal antibody (1:1000, AF7017, Affbiotech, China).

Techniques: Expressing, Western Blot, Staining, Transmission Assay, Electron Microscopy